The company aims to build upon trials performed in NSW and Victoria and will involve partially automated vehicles.
Brisbane City Council, the Centre for Accident Research and Road Safety Queensland, the Department for Transport and Main Roads, Queensland Police and the Royal Automobile Club of Queensland will advise the trial.
Transurban will also work closely with car manufacturers Audi, BMW, Hyundai, Jaguar Land Rover, Mercedes Benz, Toyota and Volvo.
The CAVs will be tested under a variety of driving conditions at different times of the day to observe how they interact with Brisbane’s motorways.
The on-road trials will begin next week and finish in late November, with results of the trial to be published in 2019.